Data/hora: 01/06/2024 15:21:09

Provedor de dados: | 80
País: | Brazil
Título: | Clostridium perfringens isolate typing by multiplex PCR
Autores: | Ahsani,MR; Mohammadabadi,MR; Shamsaddini,MB.
Data: | 2010-01-01
Ano: | 2010
Palavras-chave: | Clostridium perfringens; Multiplex PCR; Biochemical tests; Genetic typing.
Resumo: | Clostridium perfringens is an important pathogen that provokes numerous different diseases. This bacterium is classified into five different types, each of which capable of causing a different disease. There are various methods for the bacterial identification, many are labor-intensive, time-consuming, expensive and also present low sensitivity and specificity. The aim of this research was to identify the different types of C. perfringens using PCR molecular method. In this study, 130 sheep-dung samples were randomly collected from areas around the city of Kerman, southeastern Iran. After processing and culturing of samples, the produced colonies were morphologically studied, gram stain test was also carried out and the genera of these bacteria were identified through biochemical tests. DNA extracted from isolated bacteria for genotyping was tested by multiplex PCR with specific primers. Based on length of synthesized fragments by PCR, toxin types and bacterial strains were detected. C. perfringens isolated types were divided as follows: 17.39% type A, 21.74% type B, 34.78% type C and 26.09% type D. It should be emphasized that, up to the present moment, C. perfringens type A has not been reported in Iran.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400006
Editor: | Centro de Estudos de Venenos e Animais Peçonhentos
Relação: | 10.1590/S1678-91992010000400006
Formato: | text/html
Fonte: | Journal of Venomous Animals and Toxins including Tropical Diseases  v.16 n.4 2010
Fonte: | info:eu-repo/semantics/openAccess