Data/hora: 06/06/2024 02:32:42

Provedor de dados: | 80
País: | Brazil
Título: | Antinociceptive effect of PnTx4(5-5), a peptide from Phoneutria nigriventer spider venom, in rat models and the involvement of glutamatergic system
Autores: | Oliveira,Camila Franco Batista; Alves,Daniela Pereira; Emerich,Bruna Luiza; Figueiredo,Suely Gomes de; Cordeiro,Marta do Nascimento; Borges,Márcia Helena; Richardson,Michael; Pimenta,Adriano Monteiro de Castro; Duarte,Igor Dimitri Gama; Lima,Maria Elena de.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | Spider toxin; Γ-ctenitoxin-Pn1a; PnTx4(5-5); Phoneutria nigriventer; Antinociception; Glutamate.
Resumo: | ABSTRACT  Background:  The venom of Phoneutria nigriventer spider is a source of numerous bioactive substances, including some toxins active in insects. An example is PnTx4(5-5) that shows a high insecticidal activity and no apparent toxicity to mice, although it inhibited NMDA-evoked currents in rat hippocampal neurons. In this work the analgesic activity of PnTx4(5-5) (renamed Γ-ctenitoxin-Pn1a) was investigated.  Methods:  The antinociceptive activity was evaluated using the paw pressure test in rats, after hyperalgesia induction with intraplantar injection of carrageenan or prostaglandin E2 (PGE2).  Results:  PnTx4(5-5), subcutaneously injected, was able to reduce the hyperalgesia induced by PGE2 in rat paw, demonstrating a systemic effect. PnTx4(5-5) administered in the plantar surface of the paw caused a peripheral and dose-dependent antinociceptive effect on hyperalgesia induced by carrageenan or PGE2. The hyperalgesic effect observed in these two pain models was completely reversed with 5 µg of PnTx4(5-5). Intraplantar administration of L-glutamate induced hyperalgesic effect that was significantly reverted by 5 μg of PnTx4(5-5) injection in rat paw.  Conclusion:  The antinociceptive effect for PnTx4(5-5) was demonstrated against different rat pain models, i.e. induced by PGE2, carrageenan or glutamate. We suggest that the antinociceptive effect of PnTx4(5-5) may be related to an inhibitory activity on the glutamatergic system.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100316
Editor: | Centro de Estudos de Venenos e Animais Peçonhentos
Relação: | 10.1590/1678-9199-jvatitd-2019-0022
Formato: | text/html
Fonte: | Journal of Venomous Animals and Toxins including Tropical Diseases  v.25 2019
Fonte: | info:eu-repo/semantics/openAccess