Data/hora: 01/06/2024 00:31:50

Provedor de dados: | 83
País: | Brazil
Título: | Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm
Autores: | Viveiros,Ana T. M.; Taffarel,Tais R.; Leal,Marcelo C.
Data: | 2014-09-01
Ano: | 2014
Palavras-chave: | CASA; Cooling; Motility; Semen; Velocity.
Resumo: | This study evaluates the effects of extender osmolality and composition on the cooling of Prochilodus lineatussperm. Sperm was diluted in six extenders: two compositions (powdered coconut water(tm) = ACP; Beltsville Thawing Solution(tm) = BTS) x three osmolalities (285, 325, and 365 mOsm/kg) plus an undiluted control, and stored at 6-8°C. Motility rate and velocities (curvilinear, straight line, and average path) were determined every other day. Osmolality did not affect the quality of cooled sperm, thus data were pooled. Motility was higher on d 0 compared to the other days and diluted samples (85-90%) yielded higher motility than control (75%). On d 2, motility was higher in BTS-diluted samples and control, but on d 4 and 6, control yielded the highest motility. Velocities decreased from d 0 to 6 in diluted samples, but not in control. On d 0, velocities were higher in BTS-diluted sperm, but, on d 2, 4, and 6, control yielded higher velocities despite of the large variation among males. Thus P. lineatussperm should be stored in BTS or without dilution, for a maximum of two days at 6-8°C. Extender osmolality between 285 and 365 mOsm/kg does not affect sperm quality during cold storage.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000300643
Editor: | Sociedade Brasileira de Ictiologia
Relação: | 10.1590/1982-0224-20130207
Formato: | text/html
Fonte: | Neotropical Ichthyology  v.12 n.3 2014
Fonte: | info:eu-repo/semantics/openAccess