Data/hora: 09/06/2024 11:41:01

Provedor de dados: | 83
País: | Brazil
Título: | Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
Autores: | Souza-Shibatta,Lenice; Ferreira,Dhiego Gomes; Oliveira,Claudio; Almeida,Fernanda Simões de; Shibatta,Oscar Akio; Sofia,Silvia Helena.
Data: | 2013-09-01
Ano: | 2013
Palavras-chave: | Brazilian Eastern basin; Catfish; Enriched genomic library; Genetic marker; SSR.
Resumo: | Thirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers developed for M. cottoides were tested in four other Pseudopimelodidae species and successful cross-species amplification was achieved for the majority of loci.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300581
Editor: | Sociedade Brasileira de Ictiologia
Relação: | 10.1590/S1679-62252013000300011
Formato: | text/html
Fonte: | Neotropical Ichthyology  v.11 n.3 2013
Fonte: | info:eu-repo/semantics/openAccess