Data/hora: 05/06/2024 18:23:31

Provedor de dados: 	83	
País: 	Brazil	
Título: 	Fresh, equilibrated and post-thaw sperm quality of Brycon orbignyanus (Valenciennes, 1850) and Prochilodus lineatus (Valenciennes, 1837) treated with either salmon GnRHa and domperidone or pituitary extract	
Autores: 	Viveiros,Ana T.M.; Gonçalves,Antonio C.S.; Nascimento,Ariane F.; Leal,Marcelo C.	
Data: 	2015-03-01	
Ano: 	2015	
Palavras-chave: 	CASA; Characiformes; Cryopreservation; Ovaprim™; Sperm motility.	
Resumo: 	The effects of reduced doses of Ovaprim™ (GnRHa + domperidone) on sperm release of Brycon orbignyanus and Prochilodus lineatus were evaluated. Furthermore, sperm quality was compared among fresh, equilibrated and post-thaw samples. Males received a single and reduced dose of Ovaprim™ (0.125 or 0.25 ml/kg); control males received pituitary extract (cPE; 3 mg/kg). Fresh sperm was evaluated for volume, concentration, seminal plasma osmolality and seminal plasma pH. Then sperm was diluted in a freezing medium, equilibrated for 15-20 min and frozen in nitrogen vapor vessel (dry-shipper). Sperm motility was analyzed during 60 s post-activation in fresh, equilibrated and post-thaw samples. Sperm quality of males treated with Ovaprim™ (both doses) were not different from that of cPE-treated males, thus these data were pooled. In B. orbignyanus, motility was higher in fresh (99%) than in equilibrated sperm (81%); post-thaw motility dropped to 42%. In P. lineatus, motility was similar in fresh (99%) and equilibrated sperm (92%); post-thaw motility was 73%. Motility decreased as a function of time post-activation, and this decrease was significant after 60 s in fresh and equilibrated sperm, and as soon as 30 s in post-thaw sperm, in both species. Ovaprim™ at 1/4 of the recommended dose can successfully replace cPE.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000100157	
Editor: 	Sociedade Brasileira de Ictiologia	
Relação: 	10.1590/1982-0224-20140066	
Formato: 	text/html	
Fonte: 	Neotropical Ichthyology  v.13 n.1 2015	
Fonte: 	info:eu-repo/semantics/openAccess