Data/hora: 11/06/2024 06:57:13

Provedor de dados: | 83
País: | Brazil
Título: | Effects of cryoprotectants and low temperatures on hatching and abnormal embryo development of Prochilodus lineatus (Characiformes: Prochilodontidae)
Autores: | Costa,Raphael S.; Souza,Fabrício M. S. de; Senhorini,José A.; Veríssimo-Silveira,Rosicleire; Ninhaus-Silveira,Alexandre.
Data: | 2017-01-01
Ano: | 2017
Palavras-chave: | Cooling; Cryopreservation; Injuries; Morphology; Toxicity.
Resumo: | ABSTRACT This study evaluated the effect of the cryoprotectants and the low temperatures on the embryonic development of Prochilodus lineatus, describing their main morphological alterations. On chilling sensitivity test, the survival rates at the twenty somites stage (20S) were 53.6% at 0ºC, and 100% in 5ºC. To test toxicity, the embryos were exposed to a graded series of 1,2-Propanediol (PROP), dimethyl sulfoxide (Me2SO4) and glycerol (GLY), terminating in a solution of high osmolarity. There was no significant difference in the embryos survival of toxicity test between series of PROP and Me2SO4 in the 6S and 20S. In the cooling protocols, were evaluated the effects of low temperature associated with cryoprotectants. At 5ºC, PROP showed survival rates above 75% in the gastrula stage (G) and above 90% in the 6S and 20S stages. High rates of abnormalities were observed, and the most recurrent were: small bodies, fins presenting uncontrolled cell growth, membrane rupture, and retraction. These results demonstrate the need to use cryoprotectant solutions, even when there is no ice nucleation, and, on the other hand, shows that high cryoprotectant concentrations promote numerous morphological lesions, compromising normal embryonic development.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000300201
Editor: | Sociedade Brasileira de Ictiologia
Relação: | 10.1590/1982-0224-20170043
Formato: | text/html
Fonte: | Neotropical Ichthyology  v.15 n.3 2017
Fonte: | info:eu-repo/semantics/openAccess