Data/hora: 10/06/2024 05:34:04

Provedor de dados: 	83	
País: 	Brazil	
Título: 	Ultrastructure of fresh and post thawed sperm of pejerrey Odontesthes bonariensis (Atheriniformes)	
Autores: 	Gárriz,Ángela; Miranda,Leandro A.	
Data: 	2013-01-01	
Ano: 	2013	
Palavras-chave: 	Cryopreservation; Morphology.	
Resumo: 	In the present study it was showed for the first time the ultrastructural morphology of O. bonariensis sperm using electron microscopy techniques. Different kinds of abnormalities were described in fresh and post thawed sperm caused by crogenic protocols. Pejerrey spermatozoon is uniflagellated and is differentiated into three parts: a small roundish head (~1.80µm in length and 1.67µm in width), a midpiece or transitional region (~1.11µm in length and 1.56µm in width), and a long tail or flagellum (~29.08µm). Samples of fresh and post thawed sperm showed evidence of morphological anomalies affecting various intracellular compartments. Spermatozoa with swollen, ruptured, or absent membranes in the head showing excess of cytoplasm, and with alteration of the spatial orientation of the mitochondria were observed. A swollen flagellum was observed containing cytoplasmic vesicles, distributed along the whole length or concentrated in a restricted part of the tail. It was also found a high level of abnormalities (60%) in frozen sperm when compared with normal sperm (18%) reflecting the damage provoked by cryopreservation procedures.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000400831	
Editor: 	Sociedade Brasileira de Ictiologia	
Relação: 	10.1590/S1679-62252013000400011	
Formato: 	text/html	
Fonte: 	Neotropical Ichthyology  v.11 n.4 2013	
Fonte: 	info:eu-repo/semantics/openAccess