Data/hora: 01/06/2024 06:47:33

Provedor de dados: 	83	
País: 	Brazil	
Título: 	Molecular assessment of Gymnotus spp. (Gymnotiformes: Gymnotidae) fishing used as live baitfish in the Tietê River, Brazil	
Autores: 	Faria-Pereira,Lilian Paula; Hilsdorf,Alexandre Wagner Silva; Albert,James; Paiva,Maria José Tavares Ranzani; Galvão,Márcia Santos Nunes.	
Data: 	2019-01-01	
Ano: 	2019	
Palavras-chave: 	Molecular identification; PCR-RFLP; Tuvira.	
Resumo: 	ABSTRACT The capture of live bait for sport fishing is an important activity for fishing communities. The main species used for this purpose are members of the genus Gymnotus, which comprises numerous species of cryptic nature that are difficult to identify based on external morphology. The aims of this work were to identify through partial sequences of the COI gene Gymnotus species fished in the Jacaré-Guaçu River, SP, and to develop a molecular diagnostic approach using PCR-RFLP to identify these species. Partial COI sequences were compared to those of other species deposited in GenBank. The sequences were assessed in the NEBCutter program to determine restriction sites in the sequence and the enzymes to be tested. Phenetic analysis performed by Neighbor-Joining method showed that the specimens sampled belong to two species preliminary identified here as G. cf. sylvius and G. cf. cuia, with G. cf. sylvius accounting for 95.2% of the individuals sampled. The enzymes NlaIII and SacI generated fragments that allowed distinguishing the Gymnotus species using PCR-RFLP. This analysis can be used to accurately identify these species, which is fundamental for monitoring Gymnotus fishing and assessing the conservation of this genetic resource.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000500205	
Editor: 	Sociedade Brasileira de Ictiologia	
Relação: 	10.1590/1982-0224-20190075	
Formato: 	text/html	
Fonte: 	Neotropical Ichthyology  v.17 n.4 2019	
Fonte: 	info:eu-repo/semantics/openAccess