Data/hora: 15/06/2024 10:20:55

Provedor de dados: | 85
País: | Argentina
Título: | Cloning and expression analysis of rubredoxin from cold-treated banana leaves
Autores: | Feng¹,RJ; Lu,LF; Yuan,KH; Cheng,P; Zhang,LL; Qi,JF; Ren,Y; Xu,XL; Zhang,XB; Zhou,LY; Zhang,YD.
Data: | 2010-12-01
Ano: | 2010
Palavras-chave: | Rubredoxin; Expression; Banana; Stress response.
Resumo: | A banana (Musa AAA, Cavendish subgroup cv. Brazil) cDNA encoding a putative rubredoxin-like protein (MaRd1) was obtained from total RNA isolated from cold-treated banana leaves using rapid amplification of cDNA ends (RACE) technique. MaRd1 cDNA contained 597 nucleotides encoding 198 amino acids in the open reading frame. MaRd1 protein showed 56% amino acid identity with that of Pyrococcus furiosus rubredoxin (P24297). A chloroplast transit peptide and a transmembrane region were detected at the N-terminus and the C-terminus, respectively, of the deduced amino acid sequence of MaRd1 gene. Southern blotting revealed the occurrence of at least two copies of MaRd1 in the banana genome. Real time quantitative RT-PCR analysis revealed that the expression of MaRd1 gene was mainly in leaves, pseudo-stems and immature fruits, while it was barely detectable in roots and flowers. Cold and salt stresses induced higher levels of MaRd1 transcript accumulation in leaves. This finding indicated a role of MaRd1 in the response to these abiotic stresses.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000200008
Editor: | Fundación Rómulo Raggio
Formato: | text/html
Fonte: | Phyton (Buenos Aires)  v.79 n.2 2010
Fonte: | info:eu-repo/semantics/openAccess