Data/hora: 14/06/2024 03:37:40

Provedor de dados: 	85	
País: 	Argentina	
Título: 	The uptake of cadmium by Allium cepa var. agrogarum L. and its effects on chromosome and nucleolar behavior in root tip cells	
Autores: 	Wang,QL; Liu,DH; Yue,JY.	
Data: 	2016-06-01	
Ano: 	2016	
Palavras-chave: 	Cadmium (Cd); Allium cepa var. agrogarum L; Chromosome aberration; Nucleoli; Nucleolar organizing region; Autophagy.	
Resumo: 	Allium cepa var. agrogarum L. seedlings are sensitive to Cd stress. We used fluorescence imaging to indicate that Cd2+ was localized in cytoplasm in the epidermis of the basal parts of root and vascular tissues after Cd treatment. The nucleoli and the cell walls were the first storage sites of Cd2+. When Cd exposure was prolonged, severe irregularly-shaped nuclei were induced. We used silver nitrate staining to analyze the effects of different concentrations (1-300 µM) of cadmium chloride on chromosome, nucleolus and nucleolus organizer regions (NORs) in root tip cells. Cd2+ induced c-mitosis, chromosome bridges, chromosome stickiness and micronuclei. More than 100 µM Cd2+ could induce nucleolar material extruded from the nucleus into the cytoplasm. The abnormal phenomena of the nucleolar cycle during mitosis induced by Cd stress contained the nucleoli, which did not exist normally and melted gradually. More silver-stained particles were seen on chromosomes at the metaphase; more silver-stained particulates were localized on the sticky chromosomes, chromosome bridges and cytoplasm. Nucleolar reconstruction was inhibited at the telophase, NORs were localized on chromosomes of c-mitosis. The LysoTracker red stained results indicated that autophagy was active. The number of autolysosomes in roots of Cd-treated plants was more than that on control plant.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000100021	
Editor: 	Fundación Rómulo Raggio	
Formato: 	text/html	
Fonte: 	Phyton (Buenos Aires)  v.85 n.1 2016	
Fonte: 	info:eu-repo/semantics/openAccess