Data/hora: 12/06/2024 01:20:57

Provedor de dados: | 86
País: | Brazil
Título: | Viability of dourado embryos cooled in different cryoprotectant solutions
Autores: | Fornari,Darci Carlos; Streit Junior,Danilo Pedro; Galo,Juliana Minardi; Guerreiro,Luís Jayme; Povh,Jayme Aparecido; Barrero,Nelson Mauricio Lopera; Oliveira,Diego de; Ribeiro,Ricardo Pereira.
Data: | 2013-08-01
Ano: | 2013
Palavras-chave: | Salminus brasiliensis; Cryopreservation; Fish embryo; Embryo preservation; Larvae eclosion.
Resumo: | The objective of this work was to evaluate the effect of different cryoprotectants on the viability of dourado (Salminus brasiliensis) embryos. Ten cryoprotectant solutions were tested. For each solution, 300 embryos were selected at the closing of the blastopore stage, and 300 more embryos were used as a negative control. After cooling (-8ºC for 6 hours), the embryos were rehydrated directly in the incubator until hatching. The best result is obtained with the cryoprotectant solution containing 9% methanol associated with 17% sucrose, resulting in a larvae hatching rate of 67.06%.
Tipo: | Info:eu-repo/semantics/report
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800060

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2013000800057
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.48 n.8 2013
Fonte: | info:eu-repo/semantics/openAccess