Data/hora: 05/06/2024 12:46:26

Provedor de dados: | 86
País: | Brazil
Título: | Bovine sperm cell motility after incubation in follicular fluid
Autores: | Vargas Júnior,Sergio Farias; Costa,Vitória Gasperin Guazzelli; Oliveira,Fernando Caetano de; Goularte,Karina Lemos; Haas,Cristina Sangoi; Gasperin,Bernardo Garziera; Lucia Júnior,Thomaz.
Data: | 2018-07-01
Ano: | 2018
Palavras-chave: | Infertility; Insemination; Intrafollicular artificial insemination; Spermatozoa.
Resumo: | Abstract: The objective of this work was to evaluate sperm cell motility after intrafollicular artificial insemination (IFAI) in vivo or after incubation in follicular fluid in vitro. In the in vivo experiment, IFAI was performed, followed by the recovery of follicular content 1 to 4 hours later, in order to assess sperm motility. In the in vitro experiment, spermatozoa from a pool of commercial frozen-thawed semen were evaluated for their kinetics after incubation for 1 or 3 hours, either pure (pool, control group) or in follicular fluid (FF). A low motility of sperm cells was observed in the FF samples, both in vitro and in vivo. In vitro, the main parameters negatively affected in the sperm cells incubated in FF, compared with the control, were: total motility (TM), progressive motility (PM), curvilinear distance, and straightness, after 1 hour of incubation; and TM, PM, average path velocity, and curvilinear velocity after 3 hours of incubation. The ovarian follicle and follicular fluid do not provide a suitable environment to maintain bovine sperm cell motility.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000700866

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/s0100-204x2018000700011
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.53 n.7 2018
Fonte: | info:eu-repo/semantics/openAccess