Data/hora: 13/06/2024 08:15:59

Provedor de dados: | 86
País: | Brazil
Título: | Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence
Autores: | Ribeiro,Carlos Alexandre Gomes; Tanure,Janaína Paula Marques; Maciel,Talles Eduardo Ferreira; Barros,Everaldo Gonçalves de.
Data: | 2013-03-01
Ano: | 2013
Palavras-chave: | Glycine max; Cultivar protection; Genetic diversity; Genotyping method; Random identity probability.
Resumo: | The objective of this work was to standardize a semiautomated method for genotyping soybean, based on universal tail sequence primers (UTSP), and to compare it with the conventional genotyping method that uses electrophoresis in polyacrylamide gels. Thirty soybean cultivars were genotypically characterized by both methods, using 13 microsatellite loci. For the UTSP method, the number of alleles (NA) was 50 (2-7 per marker) and the polymorphic information content (PIC) ranged from 0.40 to 0.74. For the conventional method, the NA was 38 (2-5 per marker) and the PIC varied from 0.39 to 0.67. The genetic dissimilarity matrices obtained by the two methods were highly correlated with each other (0.8026), and the formed groups were coherent with the phenotypic data used for varietal registration. The 13 markers allowed the distinction of all analyzed cultivars. The low cost of the UTSP method, associated with its high accuracy, makes it ideal for the characterization of soybean cultivars and for the determination of genetic purity.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000300005

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2013000300005
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.48 n.3 2013
Fonte: | info:eu-repo/semantics/openAccess