Data/hora: 09/06/2024 12:37:15

Provedor de dados: | 86
País: | Brazil
Título: | Alternative genotyping method for the single nucleotide polymorphism A2959G (AF159246) of the bovine CAST gene
Autores: | Curi,Rogério Abdallah; Chardulo,Luis Artur Loyola; Silveira,Antônio Carlos; Oliveira,Henrique Nunes de.
Data: | 2008-05-01
Ano: | 2008
Palavras-chave: | Calpastatin gene; Meat texture; PCR-RFLP; SNP.
Resumo: | The objective of this work was to genotype the single nucleotide polymorphism (SNP) A2959G (AF159246) of bovine CAST gene by PCR-RFLP technique, and to report its use for the first time. For this, 147 Bos indicus and Bos taurus x Bos indicus animals were genotyped. The accuracy of the method was confirmed through the direct sequencing of PCR products of nine individuals. The lowest frequency of the meat tenderness favorable allele (A) in Bos indicus was confirmed. The use of PCR-RFLP for the genotyping of the bovine CAST gene SNP was shown to be robust and inexpensive, which will greatly facilitate its analysis by laboratories with basic structure.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000500014

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2008000500014
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.43 n.5 2008
Fonte: | info:eu-repo/semantics/openAccess