Data/hora: 13/06/2024 03:40:35

Provedor de dados: | 86
País: | Brazil
Título: | Optimal use of SSR markers for varietal identification of upland cotton
Autores: | Pereira,Guilherme da Silva; Cazé,Ana Luíza Ramos; Silva,Michelle Garcia da; Almeida,Vanessa Cavalcante; Magalhães,Fernanda Oliveira da Cunha; Silva Filho,João Luís da; Barroso,Paulo Augusto Vianna; Hoffmann,Lúcia Vieira.
Data: | 2015-07-01
Ano: | 2015
Palavras-chave: | Gossypium hirsutum; Cultivar discrimination; Intraspecific polymorphism; Microsatellite.
Resumo: | Abstract: The objective of this work was to identify polymorphic simple sequence repeat (SSR) markers for varietal identification of cotton and evaluation of the genetic distance among the varieties. Initially, 92 SSR markers were genotyped in 20 Brazilian cotton cultivars. Of this total, 38 loci were polymorphic, two of which were amplified by one primer pair; the mean number of alleles per locus was 2.2. The values of polymorphic information content (PIC) and discrimination power (DP) were, on average, 0.374 and 0.433, respectively. The mean genetic distance was 0.397 (minimum of 0.092 and maximum of 0.641). A panel of 96 varieties originating from different regions of the world was assessed by 21 polymorphic loci derived from 17 selected primer pairs. Among these varieties, the mean genetic distance was 0.387 (minimum of 0 and maximum of 0.786). The dendrograms generated by the unweighted pair group method with arithmetic average (UPGMA) did not reflect the regions of Brazil (20 genotypes) or around the world (96 genotypes), where the varieties or lines were selected. Bootstrap resampling shows that genotype identification is viable with 19 loci. The polymorphic markers evaluated are useful to perform varietal identification in a large panel of cotton varieties and may be applied in studies of the species diversity.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015000700571

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2015000700007
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.50 n.7 2015
Fonte: | info:eu-repo/semantics/openAccess