Data/hora: 06/06/2024 17:22:49

Provedor de dados: | 86
País: | Brazil
Título: | Identification of reference genes for expression analysis by real-time quantitative PCR in drought-stressed soybean
Autores: | Stolf-Moreira,Renata; Lemos,Eliana Gertrudes de Macedo; Abdelnoor,Ricardo Vilela; Beneventi,Magda Aparecida; Rolla,Amanda Alves Paiva; Pereira,Selma dos Santos; Oliveira,Maria Cristina Neves de; Nepomuceno,Alexandre Lima; Marcelino-Guimarães,Francismar Corrêa.
Data: | 2011-01-01
Ano: | 2011
Palavras-chave: | Glycine max; Expression stability; RT-qPCR; Water deficit.
Resumo: | The objective of this work was to validate, by quantitative PCR in real time (RT-qPCR), genes to be used as reference in studies of gene expression in soybean in drought-stressed trials. Four genes commonly used in soybean were evaluated: Gmβ-actin, GmGAPDH, GmLectin and GmRNAr18S. Total RNA was extracted from six samples: three from roots in a hydroponic system with different drought intensities (0, 25, 50, 75 and 100 minutes of water stress), and three from leaves of plants grown in sand with different soil moistures (15, 5 and 2.5% gravimetric humidity). The raw cycle threshold (Ct) data were analyzed, and the efficiency of each primer was calculated for an overall analysis of the Ct range among the different samples. The GeNorm application was used to evaluate the best reference gene, according to its stability. The GmGAPDH was the least stable gene, with the highest mean values of expression stability (M), and the most stable genes, with the lowest M values, were the Gmβ-actin and GmRNAr18S, when both root and leaves samples were tested. These genes can be used in RT-qPCR as reference gene for expression analysis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000100008

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2011000100008
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.46 n.1 2011
Fonte: | info:eu-repo/semantics/openAccess