Data/hora: 05/06/2024 17:12:40

Provedor de dados: | 88
País: | Brazil
Título: | Chemical composition, acetylcholinesterase inhibitory and antifungal activities of Pera glabrata (Schott) Baill. (Euphorbiaceae)
Autores: | Cardoso-Lopes,Elaine Monteiro; Paula,Daisy Maria Bentes de; Barbo,Fausto Erritto; Souza,Amanda de; Blatt,Cecília Terumi Teradaira; Torres,Luce Maria Brandão; Young,Maria Cláudia Marx.
Data: | 2009-12-01
Ano: | 2009
Palavras-chave: | 9-hydroxy-4-megastigmen-3-one; Biological activities; Caffeine; Euphorbiaceae.
Resumo: | Pera glabrata (Schott) Baill. was selected for this study after showing a preliminary positive result in a screening of Atlantic Forest plant species in the search for acetylcholinesterase inhibitors and antifungal compounds. The bioassays were conducted with crude ethanol extract of the leaves using direct bioautography method for acetylcholinesterase and antifungal activities. This extract was partitioned with hexane, chloroform and ethyl acetate solvents. The active chloroform fraction was submitted to silica gel chromatography column affording 12 groups. Caffeine, an alkaloid, which showed detection limits of 0.1 and 1.0 µg for anticholinesterasic and antifungal activities, respectively, was isolated from group nine. After microplate analyses, only groups four, nine, 10, 11 and 12 showed acetylcholinesterase inhibitory activity of 40% or higher. The group 12 was purified by preparative layer chromatography affording four sub-fractions. Two sub-fractions from this group were analyzed by gas chromatography-mass spectrometry and gas chromatography-flame ionization detector. The first sub-fraction showed anticholinesterasic activity and contained two major compounds: 9-hydroxy-4-megastigmen-3-one (84%) and caffeine (6%). The second sub-fraction presented five major compounds identified as 9-hydroxy-4-megastigmen-3-one, isololiolide, (-) loliolide, palmitic acid and lupeol and did not show activity.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042009000400019
Editor: | Sociedade Botânica de São Paulo
Relação: | 10.1590/S0100-84042009000400019
Formato: | text/html
Fonte: | Brazilian Journal of Botany  v.32 n.4 2009
Fonte: | info:eu-repo/semantics/openAccess