Data/hora: 06/06/2024 02:27:22

Provedor de dados: | 89
País: | Brazil
Título: | Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
Autores: | Oliveira,GH; Berchieri Jr,A; Montassier,HJ.
Data: | 2006-03-01
Ano: | 2006
Palavras-chave: | Chicken; ELISA; Enzyme; Salmonella sp; Serology.
Resumo: | This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100008
Editor: | Fundação APINCO de Ciência e Tecnologia Avícolas
Relação: | 10.1590/S1516-635X2006000100008
Formato: | text/html
Fonte: | Brazilian Journal of Poultry Science  v.8 n.1 2006
Fonte: | info:eu-repo/semantics/openAccess