Data/hora: 12/06/2024 13:31:12

Provedor de dados: 	89	
País: 	Brazil	
Título: 	Evaluation of a commercial ELISA kit (IDEXX) to differentiate AI virus-infected poultry from AI-vaccinated poultry (DIVA)	
Autores: 	Talazadeh,F; Mayahi,M; Seifi,M; Pourmehdi,M.	
Data: 	2014-06-01	
Ano: 	2014	
Palavras-chave: 	Avian influenza virus (AIV); DIVA; Commercial ELISA.	
Resumo: 	Vaccination programs for the control of avian influenza (AI) in poultry have limitations due to the problem of differentiating between vaccinated and virus-infected birds. The use of AI vaccination in poultry would have greater worldwide acceptance if reliable tests that clearly discriminated between naturally infected and vaccinated-only animals (DIVA) were available. In this study, to differentiate avian influenza virus (AIV)-infected chickens vs. chickens immunized with inactivated avian influenza virus, a commercial ELISA Kit (IDEXX) was used. Using HI as the reference method, the sensitivity of the commercial ELISA kit was 100% and its specificity was 100%. In the present study, we demonstrated that, 3 weeks after infection or vaccination, sera from both infected and vaccinated groups were tested positive and the difference in mean optical density between vaccinated and challenged birds, as detected by a commercial ELISA kit (IDEXX), was very small. Therefore, this assay cannot distinguish infected from vaccinated poultry.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000200010	
Editor: 	Fundação APINCO de Ciência e Tecnologia Avícolas	
Relação: 	10.1590/1516-635x160273-78	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Poultry Science  v.16 n.2 2014	
Fonte: 	info:eu-repo/semantics/openAccess