Data/hora: 09/06/2024 13:50:21

Provedor de dados: 	90	
País: 	Brazil	
Título: 	Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species	
Autores: 	Florentino,Ligiane Aparecida; Sousa,Pedro Martins de; Silva,Jacqueline Savana; Silva,Karina Barroso; Moreira,Fatima Maria de Souza.	
Data: 	2010-08-01	
Ano: 	2010	
Palavras-chave: 	Biological N2 fixation; Selection; Inoculant strains; Phenotypic diversity; Genotypic diversity.	
Resumo: 	The genetic diversity of ten Bradyrhizobium strains was evaluated for tolerance to high temperatures, to different salinity levels and for the efficiency of symbiosis with cowpea plants (Vigna unguiculata (L.) Walp.). Eight of these strains were isolated from nodules that appeared on cowpea after inoculation with suspensions of soil sampled from around the root system of Sesbania virgata (wand riverhemp) in ecosystems of South Minas Gerais. The other two strains used in our analyses as references, were from the Amazon and are currently recommended as cowpea inoculants. Genetic diversity was analyzed by amplifying repetitive DNA elements with the BOX primer, revealing high genetic diversity with each strain presenting a unique band profile. Leonard jar assays showed that the strains UFLA 03-30 and UFLA 03-38 had the highest N2-fixing potentials in symbiosis with cowpea. These strains had more shoot and nodule dry matter, more shoot N accumulation, and a higher relative efficiency than the strains recommended as inoculants. All strains grew in media of pH levels ranging from 4.0 to 9.0. The strains with the highest N2-fixing efficiencies in symbiosis with cowpea were also tolerant to the greatest number of antibiotics. However, these strains also had the lowest tolerance to high salt concentrations. All strains, with the exceptions of UFLA 03-84 and UFLA 03-37, tolerated temperatures of up to 40 ºC. The genetic and phenotypic characteristics of the eight strains isolated from soils of the same region were highly variable, as well as their symbiotic efficiencies, despite their common origin. This variability highlights the importance of including these tests in the selection of cowpea inoculant strains.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000400011	
Editor: 	Sociedade Brasileira de Ciência do Solo	
Relação: 	10.1590/S0100-06832010000400011	
Formato: 	text/html	
Fonte: 	Revista Brasileira de Ciência do Solo  v.34 n.4 2010	
Fonte: 	info:eu-repo/semantics/openAccess