Data/hora: 02/06/2024 03:33:24

Provedor de dados: | 93
País: | Brazil
Título: | Isolation and partial purification of beta-galactosidases from cotyledons of two cowpea cultivars
Autores: | ENÉAS-FILHO,JOAQUIM; BARBOSA,GISLAINY KARLA DA COSTA; SUDÉRIO,FABRÍCIO BONFIM; PRISCO,JOSÉ TARQUÍNIO; GOMES-FILHO,ENÉAS.
Data: | 2001-01-01
Ano: | 2001
Palavras-chave: | Cotyledons; Isozymes; Vigna unguiculata; Vita 3 and Vita 5 cultivars; Salt tolerance.
Resumo: | Three isoforms of beta-galactosidases were isolated and partially purified from the cotyledons of quiescent seeds of Vita 3 and Vita 5 cowpea [Vigna unguiculata (L.) Walp.] cultivars differing in water and salt stress tolerance. The purification procedure consisted of ammonium sulfate fractionation, acid precipitation, ion exchange chromatography through DEAE-sephadex and affinity chromatography through Lactosyl-sepharose columns. The three isoforms isolated from the two cultivars showed the same chromatographic patterns, same optimum of temperature for enzyme activity assay (60ºC), identical thermal stability up to 50°C, and similar pH optima (3-4). However, they differed from each other in sensitivity towards metal ions and certain chemical agents presents in the assay medium. The results have shown that the observed differences in beta-galactosidases from the cotyledons of quiescent seeds were not sufficient to relate them to stress tolerance.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000300001
Editor: | Sociedade Brasileira de Fisiologia Vegetal
Relação: | 10.1590/S0103-31312001000300001
Formato: | text/html
Fonte: | Revista Brasileira de Fisiologia Vegetal  v.13 n.3 2001
Fonte: | info:eu-repo/semantics/openAccess