Data/hora: 11/06/2024 12:42:14

Provedor de dados: | 94
País: | Brazil
Título: | Induction of somatic embryogenesis in immature seeds of guavatree cv. Paluma
Autores: | Moura,Elisa Ferreira; Motoike,Sérgio Yoshimitsu.
Data: | 2009-06-01
Ano: | 2009
Palavras-chave: | Tissue culture; Growth regulators; Myrtaceae.
Resumo: | The biotechnological techniques may help solve many problems of guava culture, such as the high perishability of fruits. Somatic embryogenesis can generate highly multiplicative cell cultures and with high regenerative potential, serving as basis for genetic transformation. The aim of this work was to obtain somatic embryogenesis of guava (Psidium guajava L.) cv. Paluma. Immature seeds were used, and they were inoculated in MS environment containing 400 mg L-1 of L-glutamine, 100 mg L-1 myo-inositol, 60 g L-1 sucrose, 100 mg L-1 ascorbic acid and supplemented with different types and concentrations of growth regulators. Embryogenic callus appeared after 37 days of culture in an environment containing 1.0 mg L-1 2.4-D + 2.0 mg L-1 2-ip, in 7% of the explants. After 65 days of culture, the treatment containing 0.5 mg L-1 CPA showed 20% of explants with direct embryos, while the treatment with 1 mg L-1 had 14% of explants with direct embryos and 7% of explants with embryogenic callus. In 66.6% of embryos regenerated with 0.5 mg L¹ CPA there was the formation of secondary embryos. The use of IASP and BAP, aiming embryogenesis proliferation, led to an increase in the cellular proliferation, but calli apparently lost their embryogenic potential.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452009000200027
Editor: | Sociedade Brasileira de Fruticultura
Relação: | 10.1590/S0100-29452009000200027
Formato: | text/html
Fonte: | Revista Brasileira de Fruticultura  v.31 n.2 2009
Fonte: | info:eu-repo/semantics/openAccess