Data/hora: 06/06/2024 12:03:41

Provedor de dados: | 94
País: | Brazil
Título: | In vitro organogenesis and genetic transformation of mandarin cultivars
Autores: | Soriano,Leonardo; Tavano,Eveline Carla da Rocha; Correa,Marcelo Favaretto; Harakava,Ricardo; Mendes,Beatriz Madalena Januzzi; Mourão Filho,Francisco de Assis Alves.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | Antimicrobial peptide; Cultivar improvement; Cytokinin; Huanglongbing; Transgenic plants.
Resumo: | Abstract  The in vitro organogenesis of Fremont (Citrus clementina x ), Citrus reticulataThomas (Citrus reticulata), and Nules (Citrus clementina) mandarins was evaluated aiming to optimize a regeneration protocol that could be applied in genetic transformation. The use of epicotyl-derived explants resulted in higher explant responsiveness and number of shoots developed per explant when compared with the use of internodal-derived explants. The highest efficiency in shoot regeneration was observed in the presence of 1 mg L-1 of BAP, regardless of the explant type and cultivar. The in vitro organogenesis protocol produced transgenic plants from three mandarin cultivars expressing attA gene under the control of phloem-specific promoters.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000200802
Editor: | Sociedade Brasileira de Fruticultura
Relação: | 10.1590/0100-29452019116
Formato: | text/html
Fonte: | Revista Brasileira de Fruticultura  v.41 n.2 2019
Fonte: | info:eu-repo/semantics/openAccess