Data/hora: 11/06/2024 16:04:49

Provedor de dados: | 94
País: | Brazil
Título: | Temporary immersion biorreators: efficient technique for the propagation of the ‘Pircinque’ strawberry
Autores: | Camargo,Samila Silva; Rufato,Leo; Magro,Maicon; Souza,André Luiz Kulkamp de.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | Fragaria Ananassa; Micropropagation; Automation; Liquid medium.
Resumo: | Abstract  The in vitro propagation technique via temporary immersion bioreactors is a tool that, through the culture in a liquid medium, allows an increase in the efficiency of seedling production. Several researches with the strawberry crop have shown greater efficiency of the system compared to the conventional process of micropropagation in solid medium. In this sense, the objective herein was to establish a protocol of multiplication and rooting of the ‘Pircinque’ strawberry, in temporary immersion bioreactors. Two distinct and independent studies were carried out, characterized by the multiplication and rooting stages of strawberry explants, newly introduced and registered in Brazil. Two culture media (MS and KNOP) were studied and, as a control treatment, the growth of the explants in solid culture medium was evaluated with the addition of 5 g L-1 of agar. Different immersion times of the culture medium were explored: five or eight times a day, for 15 minutes. The study was composed of the culture medium and immersion time factors, as well as the control (solid) treatment. It was verified that the use of temporary immersion bioreactors system is an efficient technique for the multiplication and rooting of explants of strawberry cv. Pircinque, when compared to the conventional method of micropropagation with the use of solid culture medium, making it possible to optimize the production of seedlings in biofactories. The MS liquid medium, in contact with explants of ‘Pircinque’ strawberry five times a day, increased the growth of the aerial part and the root system.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000101001
Editor: | Sociedade Brasileira de Fruticultura
Relação: | 10.1590/0100-29452019102
Formato: | text/html
Fonte: | Revista Brasileira de Fruticultura  v.41 n.1 2019
Fonte: | info:eu-repo/semantics/openAccess