Data/hora: 16/06/2024 06:37:24

Provedor de dados: 	94	
País: 	Brazil	
Título: 	MOLECULAR DIAGNOSIS OF Guignardia citricarpa IN ASYMPTOMATIC SWEET ORANGE TISSUE	
Autores: 	FAGANELLO,FERNANDA DE SILLOS; CARRER FILHO,RENATO; DIAS,VANESSA DUARTE; MORELLO,REGINA MELO SARTORI COELHO; CUNHA,MARCOS GOMES DA.	
Data: 	2017-01-01	
Ano: 	2017	
Palavras-chave: 	Citriculture; Molecular diagnosis; Phyllosticta citricarpa.	
Resumo: 	ABSTRACT  Citrus black spot, a fungal disease caused by the quarantine fungus Guignardia citricarpa, restricts the exportation of fresh fruit to countries in the European Union. The occurrence of latent infections and the time required for diagnosis using conventional methods have brought about the need to validate fast, efficient and reproducible molecular techniques to detect the pathogen in asymptomatic tissue. As such, this study aims to detect G. citricarpa in the symptomatic fruit and asymptomatic leaf tissue of sweet oranges by conventional and real-time polymerase chain reaction (PCR). Specificity and limit of detection (LOD) were assessed in tissue samples of fruit lesions and asymptomatic leaves. Low concentrations of the fungus were found in asymptomatic leaves. Under these conditions, real-time PCR proved to be viable, reproducible and highly sensitive to detection of the pathogen.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000400404	
Editor: 	Sociedade Brasileira de Fruticultura	
Relação: 	10.1590/0100-29452017518	
Formato: 	text/html	
Fonte: 	Revista Brasileira de Fruticultura  v.39 n.4 2017	
Fonte: 	info:eu-repo/semantics/openAccess