Data/hora: 10/06/2024 03:37:52

Provedor de dados: | 95
País: | Brazil
Título: | Genetic purity certificate in seeds of hybrid maize using molecular markers
Autores: | Salgado,Kalinka Carla Padovani de Carvalho; Vieira,das Graças Guimarães Carvalho; Von Pinho,Édila Vilela de Resende; Guimarães,Cláudia Teixeira; Von Pinho,Renzo Garcia; Souza,Lourenço Viana de.
Data: | 2006-04-01
Ano: | 2006
Palavras-chave: | Zea mays; Microsatellites markers; Isoenzymatic markers.
Resumo: | One of the main features that confer high quality to the seed is its genetic purity, in which one of the major causes of contamination is the self-pollination of the female parent. Up to date, there is no accurate and fast methods for detecting such contamination. Thus, this work was carried out to certify the genetic purity in seeds of hybrid maize using different biochemical and DNA-based markers. Two single-cross hybrids and their parental lines derived from the maize breeding program at UFLA were evaluated by isoenzymatic pattern of alcohol dehydrogenase (ADH), esterase (EST), acid phosphatase (ACP), glutamate-oxaloacetate transaminase (GOT), malate dehydrogenase (MDH), isocitrate dehydrogenase (IDH), phosphoglucomutase (PGM), 6-phosphoglucomate dehydrogenase (PGDH), catalase (CAT) and ß-glucosidade (ßGLU) and by microsatellites markers. The enzymatic systems that were able to distinguish the hybrids from their parental line were the catalase, the isocitrate dehydrogenase and the esterase. The esterase showed a Mendelian segregation pattern for UFLA 8/3 hybrid, that enables a safer genetic purity certificate. Microsatellites were able to differentiate the hybrid lines and the respective parental lines. Moreover, this technique was fast, precise and without environment effects. For microsatellites, the amplification pattern was identical when young leaves or seeds were used as DNA source. The possibility of using seeds as DNA source would accelerate and facilitate the role process of the genetic purity analysis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222006000100024
Editor: | Associação Brasileira de Tecnologia de Sementes
Relação: | 10.1590/S0101-31222006000100024
Formato: | text/html
Fonte: | Revista Brasileira de Sementes  v.28 n.1 2006
Fonte: | info:eu-repo/semantics/openAccess