Data/hora: 05/06/2024 09:17:42

Provedor de dados: | 96
País: | Brazil
Título: | Cloning and sequencing of c-DNA encoding N-methyl-D-aspartate receptor subunit-1 in the hypothalamus of male sheep
Autores: | Ruiz,Ana Zuley.
Data: | 2014-04-01
Ano: | 2014
Palavras-chave: | Cloning; NMDA receptor; Ovis aries; Ram; Sequencing.
Resumo: | The objective of the present study was to report the molecular cloning and determination of the sequences N-methyl-D-aspartate subunit-1 receptor (NMDAR-1) in the hypothalamus of sheep. The re-amplified DNA template for NMDA -1 from the hypothalamus of male sheep was cloned using pBluescript-Sk-plasmids (pBSK, 2958 bp). Purified plasmids containing the NMDA receptor c-DNA were sequenced using the dye-terminator chemistry at the University of Nebraska-Lincoln, Nebraska, USA. Results were entered into the National Center for Biotechnology Web site as accession number AY434689. The sequence of Ovis aries (sheep) NMDAR-1 mRNA from hypothalamus of male sheep has 94-97% homology with Homo sapiens, 94-100% homology with Sus scrofa, and 88-90% homology with Rattus norvegicus NMDAR-1 mRNA sequence, and 94-97% homology with Homo sapiens glutamate receptor and transcript variant NR1-2 and NR1-1 mRNA sequence. These results show high evolutionary conservation of NMDA receptor subunit-1 across species.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000400183
Editor: | Sociedade Brasileira de Zootecnia
Relação: | 10.1590/S1516-35982014000400004
Formato: | text/html
Fonte: | Revista Brasileira de Zootecnia  v.43 n.4 2014
Fonte: | info:eu-repo/semantics/openAccess