Data/hora: 01/06/2024 01:13:19

Provedor de dados: 	96	
País: 	Brazil	
Título: 	Genetic diversity of four cattle breeds using microsatellite markers	
Autores: 	Machado,Marco Antonio; Schuster,Ivan; Martinez,Mário Luiz; Campos,Ana Lúcia.	
Data: 	2003-02-01	
Ano: 	2003	
Palavras-chave: 	Bovine; DNA; Genetic diversity; Marker; Microsatellite.	
Resumo: 	Four cattle breeds (Gyr, Nellore, Guzerat and Holstein) were analyzed by amplification of genomic DNA using microsatellite loci to evaluate the genetic diversity within and among them. DNA samples of 18 animals from each breed were collected to access the genetic content of them. Allele frequencies were calculated and used to generate a Nei's genetic distance matrix what was used to build a dendrogram following UPGMA clustering. As expected, Holstein breed was the most distinct from the other breeds: 1.15 in relation to Gyr, 1.12 in relation to Nellore and 0.94 in relation to Guzerat. The closest genetic distance was 0.25 between Guzerat and Nellore. A total of 64 alleles in all four breeds were detected using nine microsatellite primers. Each breed showed 53% of the total number of alleles. The average number of alleles per locus was 7.11 ± 3.21. The most informative locus was BMS1237 with 53% of observed heterozygosity and the least informative locus was BMS3004 with 12% only. The average heterozygosity detected for the nine loci were 35% and the expected value for Hardy-Weinberg equilibrium was 53%. This low heterozygosity suggests a high endogamy level among the animals sampled within each breed.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982003000100012	
Editor: 	Sociedade Brasileira de Zootecnia	
Relação: 	10.1590/S1516-35982003000100012	
Formato: 	text/html	
Fonte: 	Revista Brasileira de Zootecnia  v.32 n.1 2003	
Fonte: 	info:eu-repo/semantics/openAccess