Data/hora: 09/06/2024 00:44:44

Provedor de dados: | 96
País: | Brazil
Título: | Genetic diversity of captive spotted paca (Agouti paca) from south east Brazil assessed by the RAPD-PCR technique
Autores: | Antunes,Karine Vieira; Machado,Théa Mírian Medeiros; Serão,Nicola Vergara Lopes; Guimarães,Simone Eliza Facione; Paiva,Samuel Rezende.
Data: | 2010-02-01
Ano: | 2010
Palavras-chave: | Animal conservation; Animal genetic resources; Biodiversity; Genetic distance; Wild animals.
Resumo: | The genetic diversity was analyzed among spotted paca (Agouti paca) from three commercial flocks located in Brazil. As the genome of this species is unknown, the RAPD-PCR technique was used. Ten primers generated sixty polymorphic bands. The among and within population genetic variability estimated by analysis of molecular variance (AMOVA) was 12.55 and 87.45%, respectively. The shortest Nei distance value was 11.76% among the Carangola (CG) and São Francisco do Glória (SF) populations. This value can be explained by the exchange of reproduction males and females between the two geographically close breeding sites. The analysis of principal components showed well defined and structured groups aggregating animals according their population of origin, with some exceptions. Lower diversity was found in the São Francisco population than in the Carangola and Castelo (CS) populations. This result suggested the variability is better conserved in breeding farms with fifty or sixty animals (CG and CS) than in the breeding farm with a dozen animals (SF). The RADP-PCR technique proved to be informative for the quantification of among and within population genetic variability of the spotted paca. The phenogram generated by UPGMA using the NTSYS-PC software from the Nei Distance, grouped CG and SF on a single branch connected to the CS, with 76 and 100% accuracy, respectively, to the bootstrap. This result was not only consistent with the historical and geographical information on flocks, but also shows the need for periodic reproductive male replacement. Future studies should be developed with co-dominant markers and include spotted paca from more distant places.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000200006
Editor: | Sociedade Brasileira de Zootecnia
Relação: | 10.1590/S1516-35982010000200006
Formato: | text/html
Fonte: | Revista Brasileira de Zootecnia  v.39 n.2 2010
Fonte: | info:eu-repo/semantics/openAccess