Data/hora: 05/06/2024 10:18:05

Provedor de dados: | 96
País: | Brazil
Título: | Feed additives can differentially modulate NF-κB (RelA/p65), IGF-1, GLUT2, and SGLT1 gene expression in porcine jejunal explants
Autores: | Silveira,Hebert; Melo,Antonio Diego Brandão; Bortoluzzi,Cristiano; Costa,Leandro Batista; Rostagno,Marcos Horácio; Schinckel,Allan Paul; Garbossa,Cesar Augusto Pospissil; Cantarelli,Vinícius de Souza.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | Chemosensors; Glucose; Piglet; RT-PCR; Swine; Trophic effects.
Resumo: | ABSTRACT The intestinal gene expression of RelA/p65 (NF-κB), insulin growth factor 1 (IGF-1), glucose transporter 2 (GLUT2), and Na+/dependent glucose transporter 1 (SGLT1) were evaluated in response to benzoic acid, yeast culture, L-glutamine, and oregano essential oil, using an ex vivo model. Six piglets weighing approximately 20 kg each were sacrificed, and their jejunum was collected and segmented into five 2-cm explants. Each explant was immersed in cell culture medium according to one of the following treatments: control (without additive), 0.5% benzoic acid, 1% yeast culture, 1% L-glutamine, and 0.015% oregano oil. Gene expression was evaluated using RT-PCR. Yeast culture up-regulated the gene expression of RelA/p65, IGF-1, GLUT2, and SGLT1 in comparison with control. In addition, jejunal exposure to L-glutamine and oregano oil increased mRNA levels of GLUT2 compared with the control treatment. Exposure to oregano oil increased intestinal SGLT1 gene expression, while benzoic acid reduced SGLT1 expression compared with the control. Feed additives can differently modulate the gene expression of immune response, gut development, and glucose absorption in jejunal explants. These findings can contribute for a better understanding of the trophic action of these feed additives into the diets to optimize animal performance.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100414
Editor: | Sociedade Brasileira de Zootecnia
Relação: | 10.1590/rbz4720180105
Formato: | text/html
Fonte: | Revista Brasileira de Zootecnia  v.47 2018
Fonte: | info:eu-repo/semantics/openAccess