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Provedor de dados:  Repositório Alice
País:  Brazil
Título:  Polyethylene glycol and polyvinylpirrolidone effect on bacterial rRNA extraction and hybridization from cells exposed to tannins.
Autores:  ARCURI, P.B.
Data:  2003-11-06
Ano:  2003
Palavras-chave:  Ruminococcus albus
Microbial ecology
Animal nutrition
Ecologia microbiana
Nutrição animal
Resumo:  In order to detect fluctuations in ruminal microbial populations due to forage tannins using 16S ribosomal RNA (rRNA) probes, recovery of intact rRNA is required. The objective of this work was to evaluate the effect of polyethylene glycol (PEG) and polyvinylpirrolidone (PVP) on extraction of bacterial rRNA, in the presence of tannins from tropical legume forages and other sources, that hybridize with oligonucleotide probes. Ruminococcus albus 8 cells were exposed to 8 g/L tannic acid or 1 g/L condensed tannins extracted from Acacia angustissima, banana (Musa sp.) skin, Desmodium ovalifolium, red grape (Vitis vinifera) skin and Inga edulis, or no tannins. Cells were rinsed with Tris buffer pH 7 containing either 8% PEG or 6% PVP prior to cell lysis. Total RNA samples rinsed with either PEG or PVP migrated through denaturing agarose gels. The 16S rRNA bands successfully hybridized with a R. albus species-specific oligonucleotide probe, regardless of tannin source. The effect of rinsing buffers on the density of 16S rRNA bands, as well as on the hybridization signals was compared. There were significant effects (P<0.01) when the controls were compared to either buffer treatments due to tannin type, buffer used and the interaction of tannin type and buffer. The significant interaction indicates the influence of tannin type on the parameters evaluated.

Tipo:  Artigo em periódico indexado (ALICE)
Idioma:  Inglês
Identificador:  25117
Editor:  Pesquisa Agropecuária Brasileira, Brasília, v.38, nº 9, p.1073-1081, set. 2003.
Relação:  Área de Informação da Sede - Artigo em periódico indexado (ALICE)

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