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Provedor de dados:  Repositório Alice
País:  Brazil
Título:  Identification of suitable adjuvant for vaccine formulation with the Neospora caninum antigen NcSRS2.
Autores:  PINHEIRO, A. F.
ROLOFF, B. C.
MOREIRA, A. da S.
BERNE, M. E. A.
ANDREOTTI, R.
LEITE, F. P. L.
Data:  2018-12-18
Ano:  2018
Palavras-chave:  Neospora Caninum
Adjuvants
Vaccines
Cytokines
Proteins
Resumo:  The parasite Neospora caninum is the main cause of abortion in cattle in many countries around the world, so a vaccine is a rational approach method for the control of the disease. An effective vaccine should be able to prevent both, the horizontal and vertical transmission of N. caninum. In this study, the immune vaccinal response of the recombinant protein rNcSRS2 of N. caninum expressed in Pichia pastoris and formulated with water-in-oil emulsion, xanthan gum, and alum hydroxide was assessed in an experimental murine model. Groups of 10 Balb/c mice were subcutaneously inoculated with two doses of prNcSRS2 twenty-one days apart. After the second immunization, four mice from each group were euthanized, and splenocytes were stimulated ex vivo with recombinant protein. The IgG dynamics were evaluated by indirect ELISA, and the splenocytes cytokines transcription by qPCR. All groups elicited specific antibodies against prNcSRS2, with the water-in-oil group showing significantly (p .05) elevated titers compared to the other groups. The prNcSRS2 protein alone did not induce a significant ex vivo splenic transcription level of IFN-c, TNF-a, IL-4, IL-10, and IL-12 cytokines, except for IL-17A, and the adjuvant associations with the prNcSRS2 protein induced different cytokine transcription profiles. The water-inoil emulsion modulated the expression of TNF-a; the xanthan gum modulated IL-4, IL-10, and IL-12; and alum hydroxide modulated IFN-c, TNF-a, IL-4, IL-10, and IL-12. In conclusion, it was found that the association of the recombinant prNcSRS2 protein with different adjuvants induced different levels of specific antibody, and a distinct splenic cytokine profile in an adjuvant-dependent manner. The mechanisms of adjuvancity activity is complex, so adjuvant formulation may help in the design of efficient vaccine to control Neosporosis.

bitstream/item/188808/1/Identification-of-suitable-adjuvant-for-vaccine.pdf
Tipo:  Artigo em periódico indexado (ALICE)
Idioma:  Inglês
Identificador:  17220

http://www.alice.cnptia.embrapa.br/alice/handle/doc/1101958
Editor:  Vaccine, v. 36, n. 9, p. 1154-1159, February 2018
Relação:  Embrapa Gado de Corte - Artigo em periódico indexado (ALICE)
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