Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Registro completo
Provedor de dados:  Repositório Alice
País:  Brazil
Título:  Bacteria isolated from abscesses of small ruminants inspected in the semiarid region of Brazil.
Autores:  SILVA, W. E. de L. e
GOLVEIA, G. V.
SÁ, M. da C. A. de
GOUVEIA, J. J. de
PEIXOTO, R. de M.
RIET-CORREA, F.
VESCHI, J. L. A.
COSTA, M. M. da
Data:  2017-03-21
Ano:  2016
Palavras-chave:  Inspeção
Isolamento
PCR
Bacteria
Mycobacterium
Caprino
Cadeia produtiva
Ovino
Produção animal
Animal production
Goat
Sheep
Resumo:  Loss in the supply chain of small ruminants owing to condemnations of carcasses in the abattoirs and slaughterhouses is common in northeastern Brazil. This study aims to identify bacterial agents, including Mycobacterium spp., in the abscesses found in the postmortem analysis of the carcasses of sheep and goats bred in northeastern Brazil. Our analysis involved 679 goats and 1,838 sheep carcasses. Abscess samples were extracted and inoculated on blood agar and Lowenstein Jensen with pyruvate or glycerol for bacterial isolation. We then performed polymerase chain reaction of the hps 65 gene; samples positive for Mycobacterium spp. were subjected to DNA sequencing. Relative frequencies of abscesses in goats and sheep were 5.44 and 3.26%, respectively. Microbiological analysis revealed 87.7% bacterial growth in the inoculated samples. Among these, Corynebacterium pseudotuberculosisrepresented 67.7% of the isolates. We observed 1.9% mycobacteria growth in the abscess samples inoculated on Lowenstein-Jensen medium. PCR of DNA extracted from abscesses samples showed amplification of 0.9% of samples. After sequencing, Mycobacterium spp. isolate was identified as M. novocastrense. C. pseudotuberculosis was the main agent responsible for the formation of abscesses in the examined animals, and we did not identify any species of the M. tuberculosis complex in the examined small ruminants.

2016
Tipo:  Artigo em periódico indexado (ALICE)
Idioma:  Inglês
Identificador:  56501

http://www.alice.cnptia.embrapa.br/handle/doc/1067340

http://ainfo.cnptia.embrapa.br/digital/bitstream/item/157865/1/Josir-2016.pdf

10.5433/1679-0359.2016v37n3p133
Editor:  Semina: Ciências Agrárias, Londrina, v. 37, n. 3, p. 1337-1344, maio/jun. 2016.
Relação:  Embrapa Semiárido - Artigo em periódico indexado (ALICE)
Fechar
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional