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Provedor de dados:  Repositório Alice
País:  Brazil
Título:  Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development.
Autores:  SILVA, D. C. da
FALAVIGNA, V. da S.
FASOLI, M.
BUFFON, V.
PORTO, D. D.
PAPPAS JÚNIOR, G. J.
PEZZOTTI, M.
PASQUALI, G.
REVERS, L. F.
Data:  2017-01-26
Ano:  2016
Palavras-chave:  Videira
Corvina
Pinot Noir
Desenvolvimento da semente
Uva
Genética
Gene
Conservação
Gene
Grapevine
Diversification of grapevine
Flower
Berry
Seed development
Resumo:  The Dof (DNA-binding with one finger) protein family spans a group of plant transcription factors involved in the regulation of several functions, such as plant responses to stress, hormones and light, phytochrome signaling and seed germination. Here we describe the Dof-like gene family in grapevine (Vitis vinifera L.), which consists of 25 genes coding for Dof. An extensive in silico characterization of the VviDofL gene family was performed. Additionally, the expression of the entire gene family was assessed in 54 grapevine tissues and organs using an integrated approach with microarray (cv Corvina) and real-time PCR (cv Pinot Noir) analyses. The phylogenetic analysis comparing grapevine sequences with those of Arabidopsis, tomato, poplar and already described Dof genes in other species allowed us to identify several duplicated genes. The diversification of grapevine DofL genes during evolution likely resulted in a broader range of biological roles. Furthermore, distinct expression patterns were identified between samples analyzed, corroborating such hypothesis. Our expression results indicate that several VviDofL genes perform their functional roles mainly during flower, berry and seed development, highlighting their importance for grapevine growth and production. The identification of similar expression profiles between both approaches strongly suggests that these genes have important regulatory roles that are evolutionally conserved between grapevine cvs Corvina and Pinot Noir.

2016
Tipo:  Artigo em periódico indexado (ALICE)
Idioma:  Inglês
Identificador:  56339

http://www.alice.cnptia.embrapa.br/handle/doc/1061878

http://ainfo.cnptia.embrapa.br/digital/bitstream/item/154037/1/Diogo-2016.pdf

10.1038/hortres.2016.42
Editor:  Horticulture Research, v. 3, p. 1-10, 2016.
Relação:  Embrapa Semiárido - Artigo em periódico indexado (ALICE)
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