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Provedor de dados:  Repositório Alice
País:  Brazil
Título:  Manipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.).
Autores:  MALABARBA, J.
BUFFON, V.
MARIATH, J. E. A.
MARASHIN, F. S.
MARGIS-PINHEIRO, M.
PASQUALI, G.
REVERS, L. F.
Data:  2019-01-07
Ano:  2018
Palavras-chave:  Plant plasmid
Grapevine
Seed
Silencing
Overexpression
VviAGL11
Resumo:  Seedlessness in grapes is a desirable trait, especially for in natura consumption. Previously, we showed that VviAGL11 is the main responsible gene for seed morphogenesis in grapevine. Here we tested the function of this gene in grapevine with the use of plant plasmids. VviAGL11 was cloned into silencing and overexpression versions of p28iIR plasmid. Reproductive grapevine bunches from di ff erent seeded and seedless cultivars were separately treated with VviAGL11 -harboring plasmids, along with controls. Plasmids were detected in leaves after a month of treatment, and berries, leaves, stems and seeds were analyzed for ectopic gene expression by RT- qPCR after 90 days of plasmid injection. Fruits from the seedless ? Linda ? treated with the VviAGL11-over- expression plasmid showed high expression levels of VviAGL11 and exhibited small seeds that were not found in the untreated control samples. Mature grapes from seeded ? Italia ? and ? Ruby ? bunches treated with the VviAGL11-silencing plasmid showed decreased VviAGL11 expression, reduced number of seeds and increased number of seed traces. The present study con fi rms that VviAGL11 is a key master regulator of seed morpho- genesis in grapevine and corroborates with the applicability of plant plasmids as promising biotechnological tools to functionally test genes in perennial plants in a rapid and confident way

bitstream/item/189886/1/Malabarba-Plant-Science.pdf
Tipo:  Artigo em periódico indexado (ALICE)
Idioma:  Inglês
Identificador:  18088

http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103200
Editor:  Plant Science, v. 269, p. 126-135, 2018.
Relação:  Embrapa Uva e Vinho - Artigo em periódico indexado (ALICE)
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