Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Registro completo
Provedor de dados:  Repositório Alice
País:  Brazil
Título:  Chromosome number and cytogenetics of Euphorbia heterophylla L.
Autores:  AARESTRUP, J. R.
KARAM, D.
FERNANDES, G. W.
Data:  2009-01-12
Ano:  2008
Palavras-chave:  Evolution
Polyploidy
Planta daninha
Resumo:  Euphorbia heterophylla L. (Euphorbiaceae) is a herbaceous species of great economic importance due to its invasive potential and consequent damage to agriculture and pasture land. For the first time, we provide information on its chromosome nwnber, morphology, and behavior of mitotic chromosomes. Seeds were germinated and submitted to four treatments to obtain metaphases: 0.5% co1chicine for 2 to 5 h, at ambient temperature; 0.5% co1chicine for 16 to 24 h; 0.0029 M 8-hydroxyquinoline (8-HQ) for 2 to 5 h at ambient temperature, and 0.0029 M 8-HQ for 16 to 24 h at 4 degrees C. The material was then fixed in methanol: acetic acid (3: I) and kept at -20 degrees C for 24 h. Roots were macerated in the enzyme solution ofFlaxzyme (TM) (NOVO FERMENT T)-distilled water (I: 40) at 34 degrees C for 2 h and later fixed again. Chromosome preparations were obtained by the dissociation of the apical meristems. The best chromosome preparations were obtained with the use of 8-HQ for 21 h 30 min at 4 degrees C. E. heterophylla showed 2n = 28 chromosomes. The short arm of the largest pair of chromosomes of the complement (pair nwnber 1) displayed a secondary constriction while the nuc1eolus was observed in the interphasic cell. Structural rearrangements were also observed in the E. heterophylla L. genome. The genomic instability associated with polyploidy may be the result of selection shaped by environmental adaptations and/or human-induced manipulation through agricultural practices.

2008
Tipo:  Artigo em periódico indexado (ALICE)
Idioma:  Inglês
Identificador:  21239

http://www.alice.cnptia.embrapa.br/handle/doc/491644

http://ainfo.cnptia.embrapa.br/digital/bitstream/item/66645/1/Chromossome-number.pdf
Editor:  Genetics and Molecular Research, Ribeirão Preto, v. 7, n. 1, p. 217-222, 2008.
Relação:  Embrapa Milho e Sorgo - Artigo em periódico indexado (ALICE)
Fechar
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional