Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Registro completo
Provedor de dados:  Repositório Alice
País:  Brazil
Título:  Polyembryony and identification of Volkamerian lemon zygotic and nucellar seedlings using RAPD.
Autores:  ANDRADE-RODRÍGUEZ, M.
VILLEGAS-MONTER, A.
CARRILLO-CASTAÑEDA, G.
GARCÍA-VELÁZQUEZ, A.
Data:  2004-09-27
Ano:  2004
Palavras-chave:  Citrus volkameriana
Rootstock
Embryo culture
Molecular markers
Polymorphism
Porta-enxerto
Cultura de embriões
Marcadores moleculares
Polimorfismo
Resumo:  The objectives of this work were to evaluate the frequency of polyembryony, and to identify zygotic and nucellar seedlings of Citrus volkameriana using RAPD. Twenty-five polyembryonic and eight monoembryonic seeds were cultivated in vitro for six months. DNA from seedlings was extracted and used in combination with five RAPD primers to identify zygotic or nucellar origin of the seedlings. Environmental conditions of the year affected significantly (P£0.05) the morphological characteristics of fruits and the number of embryos per seed. Polyembryonic seeds ranged from 30.9%, 44.8% to 54.4% over three years. Morphological characteristic was not correlated with polyembryony. In vitro culture enable all embryos of each seed to grow, favoring the percentage of seedlings identified as zygotic. In polyembryonic and monoembryonic seeds, 25.9% and 87.5% of the seedlings, respectively, were sexually originated. In polyembryonic seeds, not all zygotic seedlings were produced by small embryos located at the micropyle.

2004
Tipo:  Artigo em periódico indexado (ALICE)
Idioma:  Inglês
Identificador:  28220

http://www.alice.cnptia.embrapa.br/handle/doc/111738

http://ainfo.cnptia.embrapa.br/digital/bitstream/item/108361/1/Polyembryony.pdf
Editor:  Pesquisa Agropecuária Brasileira, Brasília, DF, v. 39, n. 6, p. 551-559, junho 2004.
Relação:  Área de Informação da Sede - Artigo em periódico indexado (ALICE)
Fechar
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional