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Provedor de dados:  Repositório Alice
País:  Brazil
Título:  Simultaneous analysis of 25 phenolic compounds in grape juice for HPLC: method validation and characterization of São Francisco Valley samples.
Autores:  NATIVIDADE, M. M. P.
CORREA, L. C.
SOUZA, S. V. C. de
PEREIRA, G. E.
LIMA, L. C. de O.
Data:  2014-01-14
Ano:  2013
Palavras-chave:  Suco de uva
Vale do São Francisco
Perfil fenólico
Composto fenológico
HPLC
Uva
Viticultura
Suco
Grapes
Resumo:  A HPLCmethod for simultaneous determination of 25 phenolic compounds in grape juicewas optimized, validated and applied in the characterization of juices produced in São Francisco Valley (SFV), Brazil. The performance characteristics of the method were established by assays with standard solutions of phenolic compounds, spiked and unspiked samples. Linearity,matrix effects, trueness, precision, detection and quantification limits were evaluated. Linearity was demonstrated in the concentration ranges tested for all phenolic compounds. Significant matrix effects were not identified for the studied compounds.Mean recoveries ranged from86.18 to 106.50%, demonstrating no lack of trueness. Precision of the method was confirmed for the 25 phenolic compounds, with acceptable repeatability relative standard deviations (from 0.71 to 9.24%) and within-reproducibility relative standard deviations (from1.34 to 9.26%) for unspiked and spiked samples. The theoretical limits of detection and quantification of themethod varied from 0.001 to 0.19 Mg mL-1 and 0.003 to 0.37 Mg mL-1, respectively. The results of the validation process showed that the proposed method is fitness for purpose. This method was able to identify simultaneously 25 phenolic compounds and had advantages such as low consumption of solvents and easy sample preparation. The phenolic profile of the grape juices from SFV varied according the grape cultivar. Phenolics of the anthocyanins and tannins class predominated in red grape juices, while in white grape juice phenolic acids and tannins were found at high concentrations.

2013
Tipo:  Artigo em periódico indexado (ALICE)
Idioma:  Inglês
Identificador:  14845

http://www.alice.cnptia.embrapa.br/handle/doc/976070

http://ainfo.cnptia.embrapa.br/digital/bitstream/item/95257/1/Mariana-Pereira-et-al-MJ-092013.pdf
Editor:  Microchemical Journal, New York, v. 110, p. 665-674, abr. 2013.
Relação:  Embrapa Uva e Vinho - Artigo em periódico indexado (ALICE)
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