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Provedor de dados:  Repositório Alice
País:  Brazil
Título:  Ovarian and follicular variables used to determine ewes with different FecGE genotypes.
Autores:  CHAVES, M. S.
LUZ, V. B.
FERREIRA-SILVA, J. C.
MELO, E. de O.
PAIVA, S. R.
BARROS, I. de
BARTOLOMEU, C. C.
AZEVEDO, H. C.
OLIVEIRA, M. A. L.
Data:  2019-09-24
Ano:  2019
Palavras-chave:  Prolificacy
GDF-9
Ovine
Polymorphism
Ovulation
Resumo:  Abstract Based on ovarian and follicular variables, there was determination of ewes with different FecGE genotypes. Based on the FecGE genotype, 65 Santa Inês ewes were assigned to three experimental groups: homozygous wild-type (n = 25; FecG+/+), mutant heterozygous (n = 27; FecG+/E) and mutant homozygous (n = 13; FecGE/E). The ewe?s ovaries were weighed and measured, then the follicles (oocyte, nucleus and nucleolus) were histologically evaluated for morphometry and morphology. Morphologically normal follicles, in the primordial and transitional stages, explained 70.18% of the variability morphological characteristics between mutant and wild-type ewes. Conducting the morphometric evaluation resulted in a more precise determination of the genotype groups when there was assessment of the primordial and secondary follicular developmental stages. The diameter of the oocyte and the oocyte nucleus of the primordial follicles explained 36.76% of the variability in follicular morphology between ewes with the mutation and those with the wildtype group. Similarly, the core diameter of oocytes in secondary follicles explained 10.63% of the variability in follicular morphology among FecGE/E, FecG+/E and FecG+/+ ewes. Thus, morphologically normal follicles in the primordial and transitional stages of development are the variables that allow for a more precise differentiation of Santa Inês ewes with the FecGE mutation. These variables may be evaluated to make more efficient the adoption of biotechniques that when conducted there is utilisation of follicles in the initial developmental stages as a physiological basis for classifying whether specific follicles are useful when conducting the techniques.

bitstream/item/202245/1/1-s2.0-S0378432019300090-main.pdf
Tipo:  Artigo em periódico indexado (ALICE)
Idioma:  Inglês
Identificador:  37843

http://www.alice.cnptia.embrapa.br/alice/handle/doc/1112410

10.1016/j.anireprosci.2019.106117
Editor:  Animal Reproduction Science, , v. 208, article 106117, 2019.
Relação:  Embrapa Recursos Genéticos e Biotecnologia - Artigo em periódico indexado (ALICE)
Formato:  7 p.
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