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Provedor de dados:  Repositório Alice
País:  Brazil
Título:  Large-scale analysis of differential gene expression in coffee genotypes resistant and susceptible to leaf miner-toward the identification of candidate genes for marker assisted-selection.
Autores:  CARDOSO, D. C.
MARTINATI, J. C.
GIACHETTO, P. F.
VIDAL, R. O.
CARAZZOLLE, M. F.
PADILHA, L.
GUERREIRO-FILHO, O.
MALUF, M. P.
Data:  2015-02-05
Ano:  2014
Palavras-chave:  Microarranjo
Plant defense
Café
Coffea arabica
Leafminers
Genes
Microarray technology
Resumo:  A successful development of herbivorous insects into plant tissues depends on coordination of metabolic processes. Plants have evolved complex mechanisms to recognize such attacks, and to trigger a defense response. To understand the transcriptional basis of this response, we compare gene expression profiles of two coffee genotypes, susceptible and resistant to leaf miner (Leucoptera coffella). A total of 22000 EST sequences from the Coffee Genome Database were selected for a microarray analysis. Fluorescence probes were synthesized using mRNA from the infested and non-infested coffee plants. Array hybridization, scanning and data normalization were performed using Nimble Scan® e ArrayStar® platforms. Genes with foldchange values +/-2 were considered differentially expressed. A validation of 18 differentially expressed genes was performed in infected plants using qRT-PCR approach.

2014
Tipo:  Artigo em periódico indexado (ALICE)
Idioma:  Inglês
Identificador:  18242

http://www.alice.cnptia.embrapa.br/handle/doc/1007801

http://ainfo.cnptia.embrapa.br/digital/bitstream/item/117310/1/Large-scale-analysis.pdf

10.1186/1471-2164-15-66
Editor:  BMC Genomics, London, v. 15, p. 1-20, 2014.
Relação:  Embrapa Informática Agropecuária - Artigo em periódico indexado (ALICE)
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