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Provedor de dados:  Inra
País:  France
Título:  A unigene catalogue of 5700 expressed genes in cassava
Autores:  Lopez, C.
Jorge, V.
Piégu, B.
Mba, C.
Cortes, D.
Restrepo, S.
Soto, M.
Laudié, M.
Berger, C.
Cooke, R.
Delseny, M.
Tohme, J.
Verdier, V.
Data:  2004
Ano:  2004
Resumo:  Two economically important characters, starch content and cassava bacterial blight resistance, were targeted to generate a large collection of cassava ESTs. Two libraries were constructed from cassava root tissues of varieties with high and low starch contents. Other libraries were constructed from plant tissues challenged by the pathogen Xanthomonas axonopodis pv. manihotis. We report here the single pass sequencing of 11 954 cDNA clones from the 5' ends, including 111 from the 3' ends. Cluster analysis permitted the identification of a unigene set of 5700 sequences. Sequence analyses permitted the assignment of a putative functional category for 37% of sequences whereas ~16% sequences did not show any significant similarity with other proteins present in the database and therefore can be considered as cassava specific genes. A group of genes belonging to a large multigene family was identified. We characterize a setof genes detected only in infected libraries putatively involved in the defense response to pathogen infection. By comparing two libraries obtained from cultivars contrasting in their starch content a group of genesassociated to starch biosynthesis and differentially expressed was identified. This is the first large cassava EST resource developed today and publicly available thus making a significant contribution to genomicknowledge of cassava.
Tipo:  Journal Article
Idioma:  Inglês
Fonte:  Plant Molecular Biology. 2004, 56 (4) : 541-554

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