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Provedor de dados:  Inra
País:  France
Título:  Voltage-dependant and glutamate-induced macroscopic ionic currents recorded with the patch-clamp technique in skeletal muscle fibers enzymatically isolated from adult honeybee.
Autores:  Collet, C.
Belzunces, L.
Data:  2006
Ano:  2006
Palavras-chave:  ABEILLE DOMESTIQUE
INSECTE SOCIAL
POLLINISATEUR
ADULTE
MUSCLE SQUELETTIQUE
FIBRE MUSCULAIRE
COURANT IONIQUE
ELECTROPHYSIOLOGIE HONEYBEE
Resumo:  Adult honeybee skeletal muscle fibers were enzymatically isolated from the metathoracic tibia (third pair of legs). Their electrophysiological properties were explored with the whole-cell patch-clamp technique. In a physiological-like extracellular solution, both inward and outward currents underlying an action potential activity were recorded in response to controlled membrane depolarizations from a resting potential of -80 mV. In an extracellular solution devoid of sodium but containing 2 mM calcium and the potassium channel blockers TEA and 4-AP, a current with an L-type calcium channel profile was recorded. The activation threshold potential for this current was ~ -40 mV and its mean maximal amplitude, reached at ~ 0 mV, was -8.5 ±1.9 A/F (n=13, mean ± S.E.M). Individual I/V curves were fitted with equation I(V)=Gmax(V-Vrev)/({1+exp[(V0.5-V)/k]}) and mean values for Gmax, Vrev, V0.5 and kwere 217 ± 35 S/F, +39.8 ± 4.1 mV, -13.7 ± 1.8 mV and 5.4 ± 0.5 mV respectively (n=13).Responses to L-glutamate, an excitatory neurotransmitter at the neuromuscular synapse of invertebrates were also recorded in the whole-cell configuration. This cell preparation appears quite suitable for patch-clamp studies, and further work will be made on the pharmacological sensitivity of ionic currents in this preparation, including their possible perturbations by insecticides compounds.
Tipo:  Journal Article
Idioma:  Inglês
Identificador:  http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20071e8542ec&uri=/notices/prodinra1/2010/12/
Fonte:  Journal of Muscle Research and Cell Motility. 2006, 27 (5-7 numéro spécial : Special issue) : 515
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