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Provedor de dados:  OMA
País:  Belgium
Título:  The nuclear rDNA region of <i>Gyrodactylus arcuatus</i> and <i>G. branchicus</i> (Monogenea: Gyrodactylidae)
Autores:  Zietara, M.S.
Arndt, A.
Geets, A.
Hellemans, B.
Volckaert, F.A.M.J.
Data:  2000
Ano:  2000
Palavras-chave:  DNA
Molecular structure
Nucleotide sequence
Gasterosteus aculeatus
Resumo:  The primary structure of the ribosomal DNA internal transcribed spacers (ITS-1 and ITS-2) and 5.8S rRNA gene were used to characterize and identify 2 monogenean species of <i>Gyrodactylus</i> living externally on the threespine stickleback (<i>Gasterosteus aculeatus</i>). The ITS region was amplified by PCR from freshwater, brackish, and marine isolates of <i>Gyrodactylus arcuatus</i> and <i>G. branchicus</i>, and the ends of the coding regions were identified by comparative alignment. No intraspecific and very low interspecific variation were observed in the 5.8S rRNA gene; high inter- and low intraspecific variation were revealed in the ITS-1 and ITS-2 regions. The morphological species identification was in all cases confirmed by the molecular identification. Intraspecifically, samples from 2 locations in the North Sea could be differentiated, but the Baltic sample resembled North Sea genotypes. Our approach offers perspectives for a multimetric genetical, morphometrical, and ecological taxonomy of the genus <i>Gyrodactylus</i>.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Relação:  info:eu-repo/semantics/altIdentifier/wos/000166184200035
Direitos:  info:eu-repo/semantics/restrictedAccess

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