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Provedor de dados:  Biological Sciences
País:  Brazil
Título:  Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation-vitrification
Autores:  Prudente, Débora de Oliveira
Paiva, Renato
Nery, Fernanda Carlota
Máximo, Wesley Pires Flausino
Silva, Luciano Coutinho
Data:  2017-05-03
Ano:  2017
Palavras-chave:  Botânica criopreservação de recursos genéticos vegetais Mangabeira
Recalcitrant species
Long-term conservation
Dehydration. Botânica
Resumo:   Hancornia speciosa is a fruitful species from Cerrado biome with high economic potential. However, the intense and disordered extractivism have caused a reduction of its population in its endemic area. In addition, seed recalcitrance negatively affects the conventional conservation of the species. Aiming to find alternatives that enable the long-term conservation of this species, the study’s objective was to assess the behavior of lateral bud’s regrowth after cryopreservation procedures by encapsulation-vitrification technique. Sodium alginate capsules containing lateral buds were pre-cultured in liquid WPM supplemented with 1.0 M glycerol, and subsequently exposed to different concentrations of sucrose (0.3; 0.75 and 1.0 M) for 24 or 48 hours. The capsules were subjected to dehydration in silica gel or airflow hood for 0, 1, 2 and 3 hours before different incubation times in PVS2 (0, 15, 30, 60 and 120 minutes) at 0°C. A high regeneration percentage of lateral buds was observed after cryopreservation of capsules treated with 0.75 M sucrose plus 1.0 M glycerol (24 hours), associated with dehydration in an airflow hood (1 hour) and immersion in PVS2 (15 minutes). Encapsulation-vitrification allowed the long-term conservation, and provided high plant material survival rates after cryopreservation of Hancornia speciosa sensitive explants. 
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620

10.4025/actascibiolsci.v39i1.31620
Editor:  Universidade Estadual De Maringá
Relação:  http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/pdf
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/12232
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/12233
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/12234
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/12235
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/12236
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/14141
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/14142
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/14143
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/31620/14144
Formato:  application/pdf
Fonte:  Acta Scientiarum. Biological Sciences; v. 39, n. 1 (2017); 87-93

Acta Scientiarum. Biological Sciences; v. 39, n. 1 (2017); 87-93

1807-863X

1679-9283
Direitos:  Direitos autorais 2017 Acta Scientiarum. Biological Sciences
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