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Provedor de dados:  Acta biol.Colomb.
País:  Colombia
Título:  A NEW WORLD MONKEY MICROSATELLITE (AP74) HIGHLY CONSERVED IN PRIMATES
Autores:  OKLANDER,LUCIANA INÉS
STEINBERG3,ELIANA RUTH
DOLORES MUDRY,MARTA
Data:  2012-04-01
Ano:  2012
Palavras-chave:  Primates
Microsatellite markers
PCR amplification
Non-invasive sampling
Genomic conservation
Genetic variability
Resumo:  Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The analysis of the nucleotide sequences permitted the evaluation of the molecular modifications experienced during the evolutionary process in primates.
Tipo:  Journal article
Idioma:  Inglês
Identificador:  http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2012000100007
Editor:  Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biología
Formato:  text/html
Fonte:  Acta Biológica Colombiana v.17 n.1 2012
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