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Provedor de dados:  Acta Botanica
País:  Brazil
Título:  In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)
Autores:  Balbuena,Tiago Santana
Kato,Massuo Jorge
Floh,Eny Iochevet Segal
Data:  2009-03-01
Ano:  2009
Palavras-chave:  Callogenesis
Cell suspension culture
Plant growth regulators
Resumo:  Piper solmsianum is a shrub from Southeast Brazil in which many biologically active compounds were identified. The aim of this work was to establish a cell suspension culture system for this species. With this in mind, petiole and leaf explants obtained from in vitro plantlets were cultured in the presence of different plant growth regulator combinations (IAA, NAA, 2,4-D and BA). Root and indirect shoot adventitious formation, detected by histological analysis, was observed. Besides the different combinations of plant growth regulators, light regime and the supplement of activated charcoal (1.5 mg.l-1) were tested for callus induction and growth. Cultures maintained in light, on a 0.2 mg.l-1 2,4-D and 2 mg.l-1 BA supplemented medium, and in the absence of activated charcoal, showed the highest calli fresh matter increment. From a callus culture, cell suspension cultures were established and their growth and metabolite accumulation studied. The achieved results may be useful for further characterization of the activated secondary metabolites pathways in in vitro systems of P. solmsianum.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Sociedade Botânica do Brasil
Relação:  10.1590/S0102-33062009000100029
Formato:  text/html
Fonte:  Acta Botanica Brasilica v.23 n.1 2009
Direitos:  info:eu-repo/semantics/openAccess

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