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Provedor de dados:  ArchiMer
País:  France
Título:  Recovery in culture of viable but nonculturable Vibrio parahaemolyticus: regrowth or resuscitation?
Autores:  Coutard, Francois
Crassous, Philippe
Droguet, Michael
Gobin, Eric
Colwell, Rita
Pommepuy, Monique
Hervio Heath, Dominique
Data:  2007-06
Ano:  2007
Palavras-chave:  Transmission electron microscopy
Scanning electron microscopy
Vibrio parahaemolyticus
VBNC state
Resumo:  The objective of this study was to explore the recovery of culturability of viable but nonculturable ( VBNC) Vibrio parahaemolyticus after temperature upshift and to determine whether regrowth or resuscitation occurred. A clinical strain of V. parahaemolyticus Vp5 was rendered VBNC by exposure to artificial seawater ( ASW) at 4 degrees C. Aliquots of the ASW suspension of cells ( 0.1, 1 and 10 ml) were subjected to increased temperatures of 20 degrees C and 37 degrees C. Culturability of the cells in the aliquots was monitored for colony formation on a rich medium and changes in morphology were measured by scanning ( SEM) and transmission ( TEM) electron microscopy. Samples of VBNC cells were fixed and examined by SEM, revealing a heterogeneous population comprising small cells and larger, flattened cells. Forty-eight hours after temperature upshift to 20 degrees C or 37 degrees C, both elongation and division by binary fission of the cells were observed, employing SEM and TEM, but only in the 10-ml aliquots. The results suggest that a portion of VBNC cells is able to undergo cell division. It is concluded that a portion of VBNC cells of V. parahaemolyticus subjected to cold temperatures remain viable. After temperature upshift, regrowth of those cells, rather than resuscitation of all bacteria of the initial inoculum, appears to be responsible for recovery of culturability of VBNC cells of V. parahaemolyticus. Nutrient in filtrates of VBNC cells is hypothesized to allow growth of the temperature-responsive cells, with cell division occurring via binary fission, but also including an atypical, asymmetric cell division.
Tipo:  Text
Idioma:  Inglês

Editor:  Nature
Formato:  application/pdf
Fonte:  The ISME Journal (1751-7362) (Nature), 2007-06 , Vol. 1 , N. 2 , P. 111-120
Direitos:  2007 Nature

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