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Provedor de dados:  ArchiMer
País:  France
Título:  Microplastics in seafood: Benchmark protocol for their extraction and characterization
Autores:  Dehaut, Alexandre
Cassone, Anne-laure
Frere, Laura
Hermabessiere, Ludovic
Himber, Charlotte
Rinnert, Emmanuel
Riviere, Gilles
Lambert, Christophe
Soudant, Philippe
Huvet, Arnaud
Duflos, Guillaume
Paul-pont, Ika
Data:  2016-08
Ano:  2016
Palavras-chave:  Microplastics
Digestion
Method
Seafood products
Tissue
Plastic integrity
Resumo:  Pollution of the oceans by microplastics (<5 mm) represents a major environmental problem. To date, a limited number of studies have investigated the level of contamination of marine organisms collected in situ. For extraction and characterization of microplastics in biological samples, the crucial step is the identification of solvent(s) or chemical(s) that efficiently dissolve organic matter without degrading plastic polymers for their identification in a time and cost effective way. Most published papers, as well as OSPAR recommendations for the development of a common monitoring protocol for plastic particles in fish and shellfish at the European level, use protocols containing nitric acid to digest the biological tissues, despite reports of polyamide degradation with this chemical. In the present study, six existing approaches were tested and their effects were compared on up to 15 different plastic polymers, as well as their efficiency in digesting biological matrices. Plastic integrity was evaluated through microscopic inspection, weighing, pyrolysis coupled with gas chromatography and mass spectrometry, and Raman spectrometry before and after digestion. Tissues from mussels, crabs and fish were digested before being filtered on glass fibre filters. Digestion efficiency was evaluated through microscopical inspection of the filters and determination of the relative removal of organic matter content after digestion. Five out of the six tested protocols led to significant degradation of plastic particles and/or insufficient tissue digestion. The protocol using a KOH 10% solution and incubation at 60 °C during a 24 h period led to an efficient digestion of biological tissues with no significant degradation on all tested polymers, except for cellulose acetate. This protocol appeared to be the best compromise for extraction and later identification of microplastics in biological samples and should be implemented in further monitoring studies to ensure relevance and comparison of environmental and seafood product quality studies.
Tipo:  Text
Idioma:  Inglês
Identificador:  http://archimer.ifremer.fr/doc/00335/44582/44469.pdf

DOI:10.1016/j.envpol.2016.05.018

http://archimer.ifremer.fr/doc/00335/44582/
Editor:  Elsevier Sci Ltd
Formato:  application/pdf
Fonte:  Environmental Pollution (0269-7491) (Elsevier Sci Ltd), 2016-08 , Vol. 215 , P. 223-233
Direitos:  2016 Elsevier Ltd. All rights reserved.

info:eu-repo/semantics/openAccess

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