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Provedor de dados:  ArchiMer
País:  France
Título:  Isolation and physiological characterization of two novel, piezophilic, thermophilic chemolithoautotrophs from a deep-sea hydrothermal vent chimney
Autores:  Takai, Ken
Miyazaki, Masayuki
Hirayama, Hisako
Nakagawa, Satoshi
Querellou, Joel
Godfroy, Anne
Data:  2009-08
Ano:  2009
Palavras-chave:  Sp. nov.
Ecological signifiance
Hydrostatic pressure
Subsurface biosphere
Base composition
High temperature
Membrane lipids
Gen. nov.
Resumo:  P>Two novel, thermophilic piezophiles, capable of chemolithoautotrophic growth, are successfully cultivated and isolated from a black smoker chimney at the TAG field (Mid Atlantic Ridge: MAR) by using a piezophilic cultivation technique. Both strains (strains 106 and 108) represent dominant cultivated populations of the microbial communities in the chimney surface habitat. Strain 106 represents typically thin, long spiral cells under the piezophilic growth condition but short bent cells under the non-piezophilic condition. It is a strictly chemolithoautotrophic gammaproteobacterium using reduced sulfur compounds as the electron donors, and nitrate and O-2 as the electron acceptors. Based on the 16S rRNA gene sequence, strain 106 would represent a novel genus of the previously uncultivated group (Symbiont Group I; a potentially novel family) within the Gammaproteobacteria, and 'Thioprofundum lithotrophica' gen. nov., sp. nov. is proposed. Strain 108 is a short, oval rod at any of the growth pressures. It is a facultative chemoautotroph, capable of both chemolithoautotrophic growth with H-2 and S oxidations and organotrophic growth with complex organics or organic acids using nitrate and O-2 as the electron acceptors. The chemolithoautotrophic growth is strictly piezophilic and under the organotrophic growth condition, it grows at conventional pressures (0.1 MPa). Strain 108 is phylogenetically distinctive from any of the previously described genera of the family Rhodobacteraceae within the Alphaproteobacteria, and 'Piezobacter thermophilus' gen. nov., sp. nov. is proposed. The piezophilic cultivation technique can be a powerful tool to isolate and characterize the previously uncultivated phylotypes in the deep-sea hydrothermal vent environments.
Tipo:  Text
Idioma:  Inglês

Editor:  Wiley / Blackwell
Formato:  application/pdf
Fonte:  Environmental Microbiology (1462-2912) (Wiley / Blackwell), 2009-08 , Vol. 11 , N. 8 , P. 1983-1997
Direitos:  2009 Wiley Blackwell Publishing

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