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Provedor de dados:  ArchiMer
País:  France
Título:  Low-molecular-weight fucoidan enhances the proangiogenic phenotype of endothelial progenitor cells
Autores:  Zemani, Faouzia
Benisvy, Danielle
Galy Fauroux, Isabelle
Lokajczyk, Anna
Colliec-jouault, Sylvia
Uzan, Georges
Fischer, Anne Marie
Boisson Vidal, Catherine
Data:  2005-10
Ano:  2005
Palavras-chave:  Fucoidan
Endothelial progenitor cells
Alpha 6
Resumo:  Endothelial progenitor cell (EPC) transplantation is a potential means of inducing neovascularization in vivo. However, the number of circulating EPC is relatively small, it may thus be necessary to enhance their proangiogenic properties ex vivo prior to injection in vivo. Fucoidan has previously been shown to potentiate in vitro tube formation by mature endothelial cells in the presence of basic fibroblast growth factor (FGF-2). We therefore examined whether fucoidan, alone or combined with FGF-2, could increase EPC proangiogenic potency in vitro. EPC exposure to 10 mu g/ml fucoidan induced a proangiogenic phenotype, including cell proliferation (p < 0.01) and migration (p < 0.01); moreover, differentiation into vascular cords occurred in the presence of FGF-2 (p < 0.01). This latter effect correlated with upregulation of the cell-surface alpha 6 integrin subunit of the laminin receptor (P < 0.05). Compared to untreated HUVEC, untreated EPC alpha 6 expression and adhesion to laminin were enhanced two-fold. Fucoidan treatment further enhanced HUVEC but not EPC adhesion to laminin. These results show that fucoidan enhances the proangiogenic properties of EPC and suggest that ex vivo cc fucoidan preconditioning of EPC might lead to increased neovascularization when injected into ischemic tissues.
Tipo:  Text
Idioma:  Inglês

Editor:  Elsevier
Formato:  application/pdf
Fonte:  Biochemical Pharmacology (0006-2952) (Elsevier), 2005-10 , Vol. 70 , N. 8 , P. 1167-1175
Direitos:  2005 Elsevier Inc. All rights reserved

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